Dystroglycan and Mitochondrial Ribosomal Protein L34 Regulate Differentiation in the Drosophila Eye
Figure 6
Df(2R)Dg248 is required for photoreceptor organization, growth and survival during pupal development.
A) An antibody, 24B10, was used to identify R cells in eye discs of 20% pupal development, mosaic for wild type and DG-deficient cells generated by FLP/FRT system. A line in (A) separates a disrupted region from a normal region. In 20 such preparations there were many small R cells (arrows, A) adjacent to patches with normal ommatidia. (B) Quantification of the lengths of lateral (apical to basal axis) membranes and (circumferential) basal membranes of R cell showed that those in disrupted regions were significantly shorter (**, P<0.01). C–E) 20% pupal development eye disc with small clones of Df(2R)Dg248 cells. Individual R-cells labeled for 24B10 (C). Df(2R)Dg248 clones are identified by a lack of GFP labeling (D). The merge (E) reveals the morphology of the Df(2R)Dg248 photoreceptors. An ommatidium composed of only Df(2R)Dg248 R cells (arrowhead) shows disorganized R cells. A single wild type R cell (arrow) has a normal morphology within an otherwise disorganized Df(2R)Dg248 ommatidium.