Parkinson's Disease-Related Protein, α-Synuclein, in Malignant Melanoma
Figure 8
Expression of α-synuclein in tissues of patients with non-melanocytic cutaneous carcinoma.
Using mouse monoclonal anti-α-synuclein antibody 4D6, we immunostained skin sections of squamous cell carcinoma (6 samples) and basal cell carcinoma (5 samples), as well as malignant melanoma (positive control) and normal skin (negative control). The sections were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG. After washing, Liquid Permanent Red substrate was used to develop the reaction and detect α-synuclein-positive cells. The sections were then counterstained with hematoxylin and analyzed by microscopy. For each malignant disease or normal skin, two immunostainings were selected and shown in this figure. Panels A and B, squamous cell carcinoma; Panels C and D, basal cell carcinoma; Panels E and F, malignant melanoma; Panels G and H, normal skin. The localization of α-synuclein is shown by the red color of Liquid Permanent Red substrate. Nuclear counterstaining is shown by the blue color of hematoxylin. Melanin pigments are brown. Scale bar indicates 50 µm.