Parkinson's Disease-Related Protein, α-Synuclein, in Malignant Melanoma
Figure 7
Expression of α-synuclein in human nevus tissues.
Using mouse monoclonal anti-α-synuclein antibody 4D6, we immunostained tissue sections of 19 nevus samples (6 compound, 11 intradermal, and 2 junctional nevus samples) (see text for identification of samples). The sections were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG. After washing, Liquid Permanent Red substrate was used to develop the reaction and detect α-synuclein-positive cells. The sections were then counterstained with hematoxylin and analyzed by microscopy. Six immunostainings are selected and shown in this figure. Panels A–C, compound nevus; Panels D–F, intradermal nevus. The localization of α-synuclein is shown by the red color of Liquid Permanent Red substrate. Nuclear counterstaining is shown by the blue color of hematoxylin. Melanin pigments are brown. Scale bar indicates 50 µm.