Parkinson's Disease-Related Protein, α-Synuclein, in Malignant Melanoma
Figure 6
Relationship of α-synuclein with melanin pigments in human malignant melanoma tissues.
To investigate the relationship of α-synuclein with melanin pigments, tissue sections of pigmented melanoma were immunostained with mouse monoclonal anti-α-synuclein antibody 4D6. The sections were analyzed by fluorescence microscopy to determine the localization of α-synuclein and by bright field microscopy to determine the localization of melanin pigments. The localization of α-synuclein is shown by the green fluorescence of Cy2 (panel c). The localization of melanin pigments is shown by the brown color in the bright field microscopy (panel a). The localization of melanin pigments is shown by the red color in the dark field (panel b) because the image of brown pigments (panel a) was inverted and the color was then converted to red (panel b). In panel d, the image of panel b is merged with panel c. Scale bar indicates 20 µm.