Parkinson's Disease-Related Protein, α-Synuclein, in Malignant Melanoma
Figure 5
Relationship of α-synuclein with MART-1 in human malignant melanoma tissues.
To investigate the relationship between α-synuclein and MART-1, tissue sections of melanoma samples (A and B) were double-immunostained with both mouse monoclonal anti-α-synuclein (4D6) antibody and rabbit monoclonal anti-MART-1 (EP1422Y) antibody. The sections were then labeled with Cy2-conjugated anti-mouse IgG for α-synuclein and Cy3-conjugated anti-rabbit IgG for MART-1. Finally, the sections were analyzed by fluorescence microscopy. The localization of α-synuclein is shown by the green fluorescence of Cy2 (panel a). The localization of MART-1 is shown by the red fluorescence of Cy3 (panel b). Their colocalization was examined by the merging of both fluorescent images (panel c). Scale bar indicates 20 µm.