Enhanced Fluorescence Imaging of Live Cells by Effective Cytosolic Delivery of Probes
Figure 5
HDF cells seeded in a pre-cast fibrin clot gel stained using Rhodamine B octadecyl ester-loaded polymersomes and subsequently monitored using fluorescent microscopy.
(a) Imaged after 7 days at the initial cell seeding area. (b) Imaged after 7 days at the interface between the initial cell seeding area and the pre-cast gel. (c) Imaged after 14 days at the initial cell seeding area. (d) Imaged after 14 days at the interface between the initial cell seeding area and the pre-cast gel. Figure bar = 0.1 mm. 3D volumetric reconstruction of HDF cells migrating within fibrin-clot gels imaged at the migration front areas (e) and at the initial cell seeding area (f). Details of single cells and their filipodia imaged at the seeding area (g) and the migration front (h). Figure bar = 0.01 mm.