Attenuation of Reactive Gliosis Does Not Affect Infarct Volume in Neonatal Hypoxic-Ischemic Brain Injury in Mice
Figure 5
Quantification of S100+ and S100+BrdU+ cells at 22 days after hypoxia-ischemia.
(A) Number of S100+ cells per optical field in ipsi- and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (GV) (n = 29) and wild-type (WT) (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. (B) Number of S100+BrdU+ cells per optical field in ipsi and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (n = 29) and wild-type (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. The numbers represent cells counted on 8 µm thick stacks of confocal images within the 450 µm×300 µm optical fields in the respective brain regions. (C) Orthogonal sections showing S100+BrdU+ cells. Data are mean ± SD. (* p<0.05, ** p<0.01). Scale bar = 15 µm.