Critical Evaluation of Gamma-Irradiated Serum Used as Feeder in the Culture and Demonstration of Putative Nanobacteria and Calcifying Nanoparticles
Figure 5
γ-Irradiation induces the degradation of serum proteins as revealed by SDS-PAGE.
Equal volumes of (A) FBS and (B) HS, either untreated (“0 kGy”) or γ-irradiated at the doses indicated, were electrophoresed on a 10%-polyacrylamide gel in denaturing and reducing conditions. γ-Irradiation produced a dose-dependent degradation of serum proteins as seen through the gradual disappearance of the main protein bands as well as the progressive protein smearing observed in lanes 2 to 7 for both gels. Solutions of HSA, BSA, and BSF, prepared in either (C) water or (D) HEPES buffer were also electrophoresed either before or after γ-irradiation at either 5 or 30 kGy. Note the significant protein profile changes seen as a result of γ-irradiation and the protein solvent used.