HOXB13, a Target of DNMT3B, Is Methylated at an Upstream CpG Island, and Functions as a Tumor Suppressor in Primary Colorectal Tumors
Figure 5
HOXB13 and TBX18 demonstrate anti-tumorignic property in colon cancer cells in vitro.
A. Western blot analysis of cell extracts prepared from RKO cells expressing Flag-tagged HOXB13 or TBX18. Analysis of cell growth by MTT assay (B), replication potential by 3H1-thymidine incorporation (C), clonogenic survival (D) of RKO cells (pool and a clone selected at random). Each assay was performed in triplicate. Single and double asterisks denote p values ≤0.05 and ≤0.01, respectively.