Negative Regulation of Bone Formation by the Transmembrane Wnt Antagonist Kremen-2
Figure 10
Increased bone formation in Krm2-deficient mice.
(A) Von Kossa/van Gieson staining of non-decalcified vertebral body sections from 24 weeks old female wildtype (Krm2+/+) and Krm2-deficient (Krm2−/−) mice (scale bars, 1 mm). (B) Fluorescent micrographs showing a higher number of calcein-labelled surfaces in Krm2-deficient vertebral bodies (scale bars, 200 µm). (C) Histomorphometric quantification of the trabecular bone volume and osteoclast surface per bone surface (OcS/BS). (D) Histomorphometric quantification of the osteoblast surface per bone surface (ObS/BS) and the bone formation rate. (E) Von Kossa/van Gieson staining of non-decalcified tibia sections from 24 weeks old female wildtype and Krm2-deficient mice (scale bars, 1 mm). (F) Histomorphometric quantification of the trabecular bone volume and the trabecular number. All bars represent mean ± SD (n = 6). Asterisks indicate statistically significant differences.