Negative Regulation of Bone Formation by the Transmembrane Wnt Antagonist Kremen-2
Figure 9
Differentially expressed genes in Col1a1-Krm2 transgenic osteoblasts.
(A) Affymetrix Gene Chip hybridization demonstrates that several well-established osteoblast differentiation markers are expressed at similar levels in osteoblasts from wildtype (mean values indicated by the dotted red line) and transgenic mice, while other genes are expressed at lower levels in the latter ones. Bars represent mean ± SD (n = 3). Asterisks indicate statistically significant differences between the relative signal intensities in wildtype and transgenic samples. (B) Affymetrix Gene Chip hybridization of wildtype osteoblasts following treatment with Dkk1 for 6 hours at day 10 of differentiation (n = 1). Shown are the Affymetrix signal intensities and the signal log ratios (SLR) for the 25 genes displaying the strongest negative regulation by Dkk1. The mean signal ratios of the Gene Chip comparison between wildtype and Col1a1-Krm2 transgenic osteoblasts are given on the right.