Significant Type I and Type III Collagen Production from Human Periodontal Ligament Fibroblasts in 3D Peptide Scaffolds without Extra Growth Factors
Figure 5
Type I and type III Collagens fluorescent immunostaining images of periodontal ligament fibroblasts on the different scaffolds after six weeks culture.
Fluorescent immunostaining with Anti-collagen type I and Alexa fluor 488 goat anti-rabbit IgG for collagen type I (green) in A1) RADA16, B1) PRG and C1) PDS, and Anti-collagen type III and Alexa fluor 594 goat anti-mouse IgG for collagen type III (red) in A2) RADA16, B2) PRG and C2) PDS. Mix ratio of designer PRG/PDS and RADA16 scaffold is 1∶9. In case of PRG and PDS, periodontal ligament fibroblasts drastically produced type I and type III collagens which were extra-cellular matrix components of periodontal ligament. The scale bar represents 100 um for all images.