RNAi Reduces Expression and Intracellular Retention of Mutant Cartilage Oligomeric Matrix Protein
Figure 5
shRNA knockdown was maintained in the presence of high levels of COMP.
COS-7 cells with and without 3B shRNA integrant were infected with a DOX-inducible MT-COMP adenovirus. DOX dosage ranging from 1 ng/ml to 10 µg/ml was used. Protein lysate was collected 48 hours after adenovirus infection. (A) Western blot analysis of COMP expression in the absence of shRNA 3B. (B) Western blot analysis of COMP expression in the presence of shRNA 3B.