RNAi Reduces Expression and Intracellular Retention of Mutant Cartilage Oligomeric Matrix Protein
Figure 3
Cells expressing MT-COMP with a COMP-directed shRNA 3B show reduction of intracellular COMP and CRT.
COS-7 cells with (I–L) and without (E–H) shRNA 3B were infected with adenoviruses expressing MT-COMP (E–L). Uninfected COS-7 cells with shRNA 3B serves as controls (A–D). Cells were grown on coverslips for 48 hours after adenoviral infection and immunostained with COMP and CRT antibodies as described in the Methods section. DAPI (blue) staining was used to localize all of the cells in the field. COMP (green in E) co-localizes (merge-yellow in H) with CRT (red in F).