Protein Tyrosine Phosphatases, TC-PTP, SHP1, and SHP2, Cooperate in Rapid Dephosphorylation of Stat3 in Keratinocytes Following UVB Irradiation
Figure 5
TC-PTP deficiency retards Stat3 dephosphorylation after UVB exposure.
(A) Western blot analysis of phosphorylated Stat3 in total cell lysates of TC-PTP-deficient primary keratinocytes. Wild-type and TC-PTP-deficient primary keratinocytes were cultured and irradiated with UVB at a dose of 800 J/m2. Cells were collected after 1 h of UVB irradiation. Western blot analysis of (B) cytoplasmic and (C) nuclear phosphorylated Stat3 in TC-PTP-deficient primary keratinocytes. Wild-type and TC-PTP-deficient primary keratinocytes were cultured and irradiated with UVB at a dose of 800 J/m2. Cells were collected after 1 h of UVB irradiation. Cytoplasmic and nuclear lysates were separately isolated. Relative levels of phosphorylated Stat3 were quantified by densitometry. Results are the mean ± standard deviation from three independent experiments.