Protein Tyrosine Phosphatases, TC-PTP, SHP1, and SHP2, Cooperate in Rapid Dephosphorylation of Stat3 in Keratinocytes Following UVB Irradiation
Figure 2
TC45, SHP1, and SHP2 rapidly dephosphorylates Stat3 after UVB irradiation in vitro mouse keratinocytes.
(A) Western blot analysis of TC45, SHP1, and SHP2 in response to UVB irradiation. Mouse primary keratinocytes were cultured and irradiated with UVB at a dose of 800 J/m2. Cells were collected at the indicated time and total cell lysates were isolated. (B) Expression analysis of PTPRT by RT-PCR. Mouse primary keratinocytes were cultured and irradiated with UVB at a dose of 800 J/m2. Cells were collected at the indicated time and total RNA was isolated. (C-F) 3PC keratinocytes were transfected with siRNA specific for TC-PTP, PTPRT, SHP1, or SHP2. Cells were collected at the indicated time after UVB irradiation (800 J/m2). Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies specific for Stat3, PY-Stat3. (C) Inhibition of TC-PTP expression by siRNA. (D) Inhibition of PTPRT expression by siRNA. (E) Inhibition of SHP1 expression by siRNA. (F) Inhibition of SHP2 expression by siRNA. a no UVB.