Permanent Occlusion of Feeding Arteries and Draining Veins in Solid Mouse Tumors by Vascular Targeted Photodynamic Therapy (VTP) with Tookad
Figure 2
Histopathology of the Tookad-VTP-treated tumor-bearing earlobe.
Hematoxilin-Eosin staining of untreated MADB106 tumor-bearing ear sections (A–C) compared to Tookad-VTP-treated tumors 24 h post treatment (D–G) are shown. A. (magnification: ×10) The untreated tissue included viable neoplastic cells which formed a small and well-demarcated mass (outlined in black) within the dermis of the pinna. The aural cartilage is identified by asterisks. The boxed area is further magnified in B (x20) and includes the epidermis (epi), dermis, and the lumen of several vascular spaces (asterisks). At higher magnifications (C, ×40) several blood vessels surrounded by neoplastic cells were identified (lumen identified with asterisks), where the endothelial cells lining them (arrowheads) were shown to be viable. D. (magnification: ×10) 24 h following Tookad-VTP, necrosis, hemorrhage, edema and vascular congestion in the tumor area were apparent (boxed in i), with only a small degree of similar findings in the surrounding tissues (boxed in ii). E. Magnified (x20) tumor area of (Di) showed diffuse necrosis and hemorrhage, no identifiable viable neoplastic cells and a necrotic epidermal layer (epi) above the tumor. The lumen of blood vessels is marked (asterisks). F. Further magnification (x40) showed the diffuse necrosis which affected all cells and included extravasated red blood cells indicating acute hemorrhage. Two blood vessels were identified (asterisks) but no viable endothelial cells were seen. G. Magnified (x40) surrounding tissues of (Dii) showed necrotic neoplastic tissue located above the aural cartilage (car). The dermis below the cartilage (der) was mildly edematous and contained dilated dermal blood vessels (asterisks). Epidermal cells (epi) and sebaceous gland cells (arrows) contained viable nuclei.