Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
Figure 2
Initial bacterial IgG expression and periplasmic extraction.
(A and B) Non-reducing and reducing immunoblot of periplasmic extract from overnight expression of 4G2 in HB2151 (HB) and BL21(DE3) (BL) E. coli strains, respectively. All blots were probed with anti-Human Fc-HRPO.