Exploiting Nanotechnologies and TRPV1 Channels to Investigate the Putative Anandamide Membrane Transporter
Figure 7
Effect of the fatty acid binding protein-4 (FABP4) inhibitor [(2′-(5-Ethyl-3,4-diphenyl-1H-pyrazol-1-yl)(1,1′-biphenyl)-3-yl)oxy]-acetic acid, 50 µM) on the cellular uptake of [14C]-anandamide in a “conventional “uptake assay carried out in TRPV1- HEK-293 cells.
The amount (cpm) of [14C]-AEA taken up by cells at 37°C subtracted of that taken up at 4° after a 90 sec incubation with the compound is shown on the y axis. Each bar indicates means ± sem of 3 independent experiments. The effect of the FABP4 inhibitor was significantly different (P<0.01) from that of vehicle (0.1% methanol).