Stanniocalcin-1 Regulates Extracellular ATP-Induced Calcium Waves in Human Epithelial Cancer Cells by Stimulating ATP Release from Bystander Cells
Figure 5
Inhibition of PLC Reduced Mechanical and ATP Induced Calcium Response.
A) Fluo-4 labeled A549 cells were mechanically stimulated in the absence (control) or presence of either a P2X inhibitor (50 µM NF023), PI-PLC pathway inhibitor (50 µM D609), or PLC inhibitor (12.5 µM U73122) t = 20 s post-injury. n = 5. B) Quantification of distance traveled by calcium wave from A at t = 20 s. Error bars = SD. * = p<0.05. n = 5. C) Fluo-4 labeled A549 cells pre-treated for 30 min with NF023, D609, or U73122 were incubated with 500 ng/mL STC1 for 10 min prior to scrape. Maximum distance of the calcium wave is displayed. Error bars = SD. * = p<0.05. n = 3.