Voreloxin Is an Anticancer Quinolone Derivative that Intercalates DNA and Poisons Topoisomerase II
Figure 6
Topoisomerase IIα knockdown reduces the G2 arrest induced by the planar voreloxin analog.
A549 cells were transfected with siRNA targeting topoisomerase IIα (Topo IIα KD), or with scrambled control siRNA, for 48 h. At 48 h, cells were treated for 16 h with a dose-titration (0.037–3 µM) of voreloxin or analog and stained with BrdU followed by flow cytometry analysis. Histograms are shown in Figure S6. The percentages of cells in the G2 phase of the cell cycle were calculated and are represented in the line graphs. No G2 arrest was observed with the nonintercalative analog (Figure S6 and Table S3). Data are representative of three independent experiments.