From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy
Figure 4
Correlative light and scanning electron microscopy.
COS-1 cells transfected with GFP-tagged MTM1 were treated with a hypo-osmotic shock for 10 min, imaged with confocal microscopy and processed for scanning-EM. (A) Confocal fluorescence, (B) bright field and (C) scanning-EM images of a GFP-positive cell on top of the reference grid. (D) Enlargement of (C). (E) The superposition of confocal (z-stack of 3.52 µm) and scanning-EM images shows that the needles do not fit with filopodia but correspond to dorsal ruffles.