A Comparison of Azacitidine and Decitabine Activities in Acute Myeloid Leukemia Cell Lines
Figure 6
Effects of AZA and DAC on cell cycle and apoptosis in KG-1a cells.
KG-1a cells were treated daily with AZA or DAC (0–3 µM) for 48 hours. (A) Cell cycle effects of AZA and DAC. Cells were stained with NIM-DAPI and quantification by flow cytometry for percentage of cells in sub-G1, G0/G1, S, and G2-M phases (normalized to 100%). (B) AZA and DAC induce apoptosis in KG-1a cells. Apoptosis was detected with flow cytometry by positive staining for Annexin V (early apoptosis) and 7-AAD (late apoptosis). (C) Protein lysates were analyzed by Western analysis for detection of PARP cleavage. α-Tubulin is shown as a protein loading control. AZA = azacitidine; DAC = decitabine.