An Engineered Yeast Efficiently Secreting Penicillin
Figure 8
Deletion of PEX3 results in reduced PEN secretion.
HpPen4 and Δpex3 HpPen4 cells were grown in a glucose-limited chemostat culture supplemented with choline as nitrogen source in the presence of 1 mM PAA and 1 mM AAA. Using spent medium of Δpex3 HpPen4 cultures a smaller halo was produced relative to HpPen4. 6 µl of spent medium of both cultures was used.