An Engineered Yeast Efficiently Secreting Penicillin
Figure 4
Multiple reaction monitoring (MRM) chromatograms from ACV in extracts of strains HpPen1 and HpPen2 cells grown in batch cultures on methanol in the presence (+AAA) or absence (-AAA) of 8 mM AAA. The data show that ACV is only present in HpPen2 cells grown in the presence of AAA (lowest panel). An MRM chromatogram from the co-eluting 13C labeled ACV internal standard is included as control (upper panel).