An Engineered Yeast Efficiently Secreting Penicillin
Figure 3
Expression of genes involved in penicillin G production in H. polymorpha.
Western blot analysis demonstrating the presence of ACVS, Sfp, IPNS, IAT and PCL in cells of the various indicated H. polymorpha strains grown in batch cultures on methanol. The blots were decorated with the indicated antibodies except for Sfp, which was produced as a His6 tagged protein and detected by anti-His6 antibodies. Per lane 20 µg of protein was loaded, except for IPNS for which 2 µg of protein was used.