Genome-Wide Identification of Transcription Start Sites, Promoters and Transcription Factor Binding Sites in E. coli
Figure 5
Mapping the TSSs of the cysK gene.
A) The oligonucleotide primer was designed to prime 97 nucleotides downstream of the ATG. B) The A and B PCR products generated with the oligonucleotide primer were separated by PAGE and purified from the gel. C) Nucleotide sequence of each PCR band. The 3′ end the nucleotide immediately before the polynucleotide tail is the TSS. D) Comparison of the nucleotide sequences obtained with the upstream region. The previously reported TSS [52] was located 34 nucleotides upstream from the ATG, while the new TSS was located at 67 nucleotides downstream.