Genome-Wide Identification of Transcription Start Sites, Promoters and Transcription Factor Binding Sites in E. coli
Figure 4
Determination of the unknown TSSs for rpsB gene.
A) Proximal and distal oligonucleotides were design to prime 4 and 67 nucleotides downstream of the ATG, respectively. B) The PCR products generated with the oligonucleotide primers were separated by PAGE and purified from the gel. C) Nucleotide sequence of each PCR band after excision from the gel. The 3′ end the nucleotide immediately before the polynucleotide tail is the TSS. D) Comparison of the nucleotide sequences obtained with upstream region of rpsB.