LKR/SDH Plays Important Roles throughout the Tick Life Cycle Including a Long Starvation Period
Figure 9
Effect of dsRNA treatment on LKR and SDH gene disruption.
A. Reverse transcription PCR analysis. B. Western blotting analysis. LKR-, SDH- and Luc- dsRNAs and PBS were injected into the each group of female H. longicornis adults. The injected ticks (15 individuals for each group) were infested on rabbits, and ticks recovered after 4 days of feeding. Lane 1, LKR dsRNA-injected ticks; lane 2, SDH dsRNA-injected ticks; lane 3, PBS-injected ticks; lane 4, firefly gene of Luciferase (Luc) dsRNA-injected ticks.