LKR/SDH Plays Important Roles throughout the Tick Life Cycle Including a Long Starvation Period
Figure 7
Histochemical detection of SDH activity in sectioned midgut and ovary.
A. In the SDH activity staining tissue, slides stained with saccharopine are labeled with “Sacch+” and control slides not incubated with saccharopine labeled with “Sacch-”. Positive reaction shows SDH specific staining as brown dots on the oocytes and digestive cells of the midgut, whereas negative reaction incubated without saccharopine shows no specific staining. Mg, midgut; DGC, digestive cell; L, lumen; Ov, Ovary; Oc, oocyte. B. IFAT; The mouse anti-IgG conjugated with Alexa 594 was used as a secondary antibody. DGC, digestive cell; L, lumen; C. Mitochondria visualization by MitoTracker probe. MitoTracker probe, midgut tissues stained with 50 nM of Mitotracker Red CMXRos. DGC, digestive cell; L, midgut lumen. Magnification, ×20.