Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases
Figure 3
Androgen-induced expression of LNCaP proteins.
A) Expression fold change of androgen-regulated proteins in mass-spectrometric quantitation compared to immunoblot assessment. Shown are the immunoblot bands and their intensities for androgen up-regulated proteins and their calculated intensity ratios as well as iTRAQ ratios and MudPIT spectral counts for the corresponding proteins. Beta-tubulin is used as a sample loading control. B) Immunofluorescence staining of AR (Red) and FASN (Green) following vehicle (ethanol) and 1 nM R1881 treatments for 48 h on LNCaP cells. Treatment was given after 48 h of androgen deprivation.