Proteomic Interrogation of Androgen Action in Prostate Cancer Cells Reveals Roles of Aminoacyl tRNA Synthetases
Figure 2
Total and androgen-regulated proteins identified from two mass-spectrometric platforms.
A) Venn diagram showing the number of total and the sub-sets of androgen-regulated proteins identified from iTRAQ MALDI TOF- MS/MS analysis. A minimum threshold of 1.25 SD from the global mean in both androgen-treated samples and not less than 1.5 SD in one of the androgen treated samples was set. B) Venn diagram showing the numbers of total and androgen-regulated LNCaP proteins identified in MudPIT (ESI ion trap- MS/MS) analysis. Proteins shown in brackets were not considered for differential analysis because of their lower spectral counts. C) Venn diagram showing the total number of proteins identified from two mass-spectrometric platforms and their overlap. D) Venn diagram showing the total number of androgen up-regulated proteins identified from two mass-spectrometric platforms and their overlap. E. Venn diagram showing the total number of androgen down-regulated proteins identified from two mass-spectrometric platforms and their overlap.