Cell Cycle-Related Cyclin B1 Quantification
Figure 4
Comparison of MeOH fixed cells to MeOH fixed-lyophilized cells.
A culture of PC3 cells was trypsinized, washed, and then divided into three equal parts. One part was solubilized for electrophoresis, one part was fixed with methanol, and the third part was fixed with methanol and then lyophilized. The methanol-fixed and lyophilized cell preparations were washed then solubilized with 23% SDS. The lanes were loaded with equal numbers of cells.