Cell Cycle-Related Cyclin B1 Quantification
Figure 2
Extraction of cyclin B1 in various lysis buffers.
Equal numbers of dissociated PC3 cells were solubilized in lysis buffer containing RIPA, Triton X-100 or SDS (see Materials and Methods) and analyzed for cyclin B1 by Western blot (A). Increasing the SDS level to 23% did not release more cyclin B1 (B). There was some variability between lanes but, based on multiple gels, it was clear that cyclin B1 was readily extracted by all methods.