mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures
Figure 5
Distribution of peroxisomes during different stages of mitosis in HeLa (upper row) and NIH3T3 cells (lower row). Peroxisomes are highlighted by mRuby-PTS. The spindle of the mitotic cells is stained with EGFP fused to a tubulin-binding protein [14]. Images were taken 24–48 h after transfection on an Olympus IX71 microscope equipped with standard FITC and TRITC filter sets. Bar: 5 µm.