Transposition-Based Method for the Rapid Generation of Gene-Targeting Vectors to Produce Cre/Flp-Modifiable Conditional Knock-Out Mice
Figure 5
Targeting strategy for the conditional inactivation of the Cdh22 gene.
(A) Restriction maps of the mouse wild type Cdh22 locus, the targeting vector, and the targeted allele. Relevant restriction sites: E, EcoRI; K, KpnI. Vertical dotted lines highlight homologous regions. (B) Southern analysis of genomic DNA from ES cells (EcoRI digestion), with the 5′-probe illustrating the 21.5 kb wild type (wt) and 10.5 kb targeted (cond) alleles. (C) Southern analysis of genomic DNA from ES cells (EcoRI digestion), with the 3′ probe illustrating the 21.5 kb wild type (wt) and 8.9 kb targeted (cond) alleles.