Sequential Metabolism of 7-Dehydrocholesterol to Steroidal 5,7-Dienes in Adrenal Glands and Its Biological Implication in the Skin
Figure 13
Metabolism of 7DHC and 7DHP by rat skin.
A. To study the skin capability to transform 7DHC to 7DHP isolated mitochondria were incubated with 7DHC (0.5 mM) in the presence (experimental) or absence (control) of isocitrate and NADPH. 7DHP: product with the same retention time and UV spectra (inset) as 7DHP standard. B. Incubation of crude skin extract with 7DHP substrate (RT 16.7 min) in the presence of isocitrate and NADPH (experimental) resulted in appearance of a 5,7-diene product (N) with RT 11.9 min, which was absent in control reaction mixture (isocitrate and NADPH were not added) (control).