Sequential Metabolism of 7-Dehydrocholesterol to Steroidal 5,7-Dienes in Adrenal Glands and Its Biological Implication in the Skin
Figure 6
Time-dependent metabolism of 7DHP is modified by trilostane (T).
Fragments of the pig adrenal glands were incubated with 7DHP (S; 0.5 mM) in the absence (A) or presence (B) of trilostane (0.1 mM) and analyzed by RP-HPLC. Boiled adrenal fragments were used as a negative control (blue). The major products of 7DHP metabolism affected by trilostane are marked by numbers. Time-dependent changes in the relative concentration of the identified products in presence (T+) or absence (T-) of trilostane were calculated using ACDLabs software and are presented in C.