Sequential Metabolism of 7-Dehydrocholesterol to Steroidal 5,7-Dienes in Adrenal Glands and Its Biological Implication in the Skin
Figure 2
Precursor-product relationship between 7DHC, hydroxy-7DHC, dihydroxy-7DHC and 7DHP defined by their sequential metabolism by rat adrenal glands.
A. Chromatogram showing metabolism of 7DHC(S) by adrenal glands. The hydroxy-7DHC (1) and dihydroxy-7DHC (2) products were collected from the HPLC and tested as substrates (see B–E). Product 3 was identified as 7-DHP based on RT, mass and UV spectra. B. Incubation of hydroxy-7DHC (1) with adrenal glands generated dihydroxy-7DHC with RT 34.8 min (2) and 7DHP with RT 15.8 min (3). C. Boiled adrenal glands served as a control for B. D. Incubation of dihydroxy-7DHC (2) with adrenal glands produced 7DHP; note the lack of S and product 1. E. Negative control for D (boiled adrenal glands ). Inserts: UV spectra of compounds 1–3.