Mycobacterium tuberculosis Rv3802c Encodes a Phospholipase/Thioesterase and Is Inhibited by the Antimycobacterial Agent Tetrahydrolipstatin
Figure 5
Hydrolysis of various phospholipid substrates by F. solani cutinase (FS), Snake venom PLA (SV), MSMEG_1403 and Rv3802, demonstrating hydrolysis of all except SM.
Thin layer chromatography using 14C-labeled DPPC = dipalmitoyl phosphatidylcholine labeled on both fatty acids (FA), AAPC = phosphatidylcholine with 14C-arachidonic acid on the sn-2, SM = sphingomyelin 14C-labeled on headgroup, PE = phosphatidylethanolamine 14C-labeled on sn-2 FA, PS = phosphatidylserine 14C-labeled on headgroup. Inset is equivalent aqueous phase of phosphatidylserine assay.