P38 MAP Kinase Signaling Is Required for the Conversion of CD4+CD25− T Cells into iTreg
Figure 5
p38 MAP kinase inhibitor SB203580 specifically inhibits the phosphorylation of p38 MAP kinase downstream target MAPKAP2.
A: CD4+CD25− T cells were cultured with TGF-beta1 (2 ng/ml) and SB203580 (10 µM), SP600125 (10 µM), or PD89059 (50 µM) for 45 min. Control cells were cultured without TGF-beta1. B: CD4+CD25− T cells were cultured for 4 hours with TGF-beta1 (2 ng/ml) and SB203580 (10 µM). P-MAPKAP2, p-ERK, p-c-jun, p-Smad 3 and actin were determined using Western blot. Results are representative of two independent experiments.