Imaging Mass Spectrometry Technology and Application on Ganglioside Study; Visualization of Age-Dependent Accumulation of C20-Ganglioside Molecular Species in the Mouse Hippocampus
Figure 4
Localization of C20-sphingosine-containing gangliosides was confirmed by MS of extracted and methyl-esterified gangliosides.
To determine the percentage of GM1/GD1 gangliosides containing the C20-species in different regions without allowing sialic acid dissociation during MS measurement, we extracted gangliosides from the dendritic region of the SR (region A, (a)) and the ML/SLM (region B, (b)). They were derivatized to methyl-esterified gangliosides. From the result of MS of underivatized gangliosides and methyl-esterified gangliosides (c), the percentage of GM1/GD1 gangliosides containing the C20-species were calculated (d). Three different mouse brain sections were used, and the data were expressed as mean±S.D. * and ** indicate P<0.05 and P<0.005, respectively, Student's t-test.