Imaging Mass Spectrometry Technology and Application on Ganglioside Study; Visualization of Age-Dependent Accumulation of C20-Ganglioside Molecular Species in the Mouse Hippocampus
Figure 3
Localization of C20-sphingosine-containing gangliosides in the hippocampal formation.
IMS at 50 µm raster step size was used to gain an overview of ganglioside distribution in different brain regions (A), and IMS at 15 µm raster size was used to study in detail the distribution pattern of gangliosides in the hippocampus (B). In both panels, schematic diagram of the brain section (a) and ion images of STs (b–c) are presented. For ions corresponding to the GD1 molecular species, we observed the ion distributions of both sodium and potassium complexes, i.e., the ions at m/z 1858 (f) and m/z 1886 (g), which correspond to the [M+Na-H]− form of C18- and C20-GD1, and those at m/z 1874 (h) and m/z 1902 (i), which correspond to the [M+K-H]− form of C18- and C20-GD1, respectively. The ion distribution patterns corresponding to the GD1-Na salts and GD1-K salts are fairly uniform for both C18- and C20- species. For GM1, m/z 1544 (d) and m/z 1572 (e), which correspond to C18- and C20-sphingosines containing GM1 respectively are shown.