Assessment of epithelial barrier integrity in cellular monolayer models using low cost, clinically approved fluorescent contrast agents

• Elena Monfort Sanchez ,

  Contributed equally to this work with: Elena Monfort Sanchez, Aaron F. Watson

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization

  Affiliation Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom

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• Aaron F. Watson ,

  Contributed equally to this work with: Elena Monfort Sanchez, Aaron F. Watson

  Roles Data curation, Formal analysis, Investigation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom

  https://orcid.org/0009-0009-9580-6923

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• Tafhima Haider,

  Roles Data curation, Investigation, Methodology, Supervision, Validation

  Affiliation Blizard Institute, Queen Mary University of London, London, United Kingdom

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• Despoina Chrysostomou,

  Roles Data curation

  Affiliation Tropical Gastroenterology and Nutrition Group, University of Zambia School of Medicine, Lusaka, Zambia

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• Maelle Storder,

  Roles Data curation, Formal analysis

  Affiliation Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom

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• Nilanjan Mandal,

  Roles Formal analysis

  Affiliation Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom

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• Paul Kelly,

  Roles Funding acquisition, Project administration, Resources

  Affiliations Blizard Institute, Queen Mary University of London, London, United Kingdom, Tropical Gastroenterology and Nutrition Group, University of Zambia School of Medicine, Lusaka, Zambia

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• Julian R. Marchesi,

  Roles Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliation Division of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, St Mary’s Hospital, Imperial College London, London, United Kingdom

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• James Avery,

  Roles Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliations Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom, School of Electronic and Electrical Engineering, University of Leeds, Woodhouse, Leeds, United Kingdom

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• Claire D. Bourke,

  Roles Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  Affiliations Blizard Institute, Queen Mary University of London, London, United Kingdom, School of Infection & Immunity, College of Veterinary and Life Sciences, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow, United Kingdom

  https://orcid.org/0000-0002-7244-0895

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• Alex J. Thompson

  Roles Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

  * E-mail: alex.thompson08@imperial.ac.uk

  Affiliation Helen Hamlyn Centre, Institute of Global Health Innovation, Department of Surgery & Cancer, Bessemer Building, Imperial College London, London, United Kingdom

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