Impact of preanalytical variables on urinary cell-free DNA quality: Centrifugation, EDTA concentration, storage and thawing strategies

• Wenjing Li ,

  Contributed equally to this work with: Wenjing Li, Shan Guo, Zongning Zhou

  Roles Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Shan Guo ,

  Contributed equally to this work with: Wenjing Li, Shan Guo, Zongning Zhou

  Roles Investigation, Methodology, Software, Validation, Writing – original draft

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Zongning Zhou ,

  Contributed equally to this work with: Wenjing Li, Shan Guo, Zongning Zhou

  Roles Methodology, Resources, Software, Visualization

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Hongwei Peng,

  Roles Project administration, Resources

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Yan Fu,

  Roles Project administration, Resources

  Affiliation Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China

  ⨯
• Cong Zou,

  Roles Project administration, Resources

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Xin Zhang,

  Roles Resources, Supervision

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  ⨯
• Shenjuan Li,

  Roles Resources, Supervision, Visualization

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Hubei Province, Wuhan, Hubei, China

  https://orcid.org/0009-0000-3978-4672

  ⨯
• Zhangyan Zhou ,

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: zn003362@whu.edu.cn (SZ); qky1009@whu.edu.cn (KQ); zhouzhangyan2009@163.com (ZZ)

  Affiliation Department of Urology, Xingshan People’s Hospital, Yichang, Hubei, China

  ⨯
• Kaiyu Qian ,

  Roles Conceptualization, Funding acquisition, Resources, Writing – review & editing

  * E-mail: zn003362@whu.edu.cn (SZ); qky1009@whu.edu.cn (KQ); zhouzhangyan2009@163.com (ZZ)

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Hubei Key Laboratory of Urological Diseases, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China

  https://orcid.org/0000-0003-4153-1265

  ⨯
• Shanshan Zhang

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: zn003362@whu.edu.cn (SZ); qky1009@whu.edu.cn (KQ); zhouzhangyan2009@163.com (ZZ)

  Affiliations Department of Biological Repositories, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China, Human Genetic Resources Preservation Centre of Wuhan University, Wuhan, Hubei, China

  https://orcid.org/0000-0002-6320-2107

  ⨯