Optimization of the Mit1C real-time PCR assay using Bio-Rad CFX96 for the detection of Cyclospora cayetanensis in agricultural waters

• Khai Truong,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America

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• Miely Suarez,

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – review & editing

  Affiliation School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America

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• Mauricio Durigan,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Division of Food and Environmental Safety, Human Foods Program, Office of Laboratory Operations and Applied Science, Office of Applied Microbiology and Technology, U.S. Food and Drug Administration, Laurel, Maryland, United States of America

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• Laura Ewing-Peeples,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Writing – review & editing

  Affiliation Division of Food and Environmental Safety, Human Foods Program, Office of Laboratory Operations and Applied Science, Office of Applied Microbiology and Technology, U.S. Food and Drug Administration, Laurel, Maryland, United States of America

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• Mark C. Jenkins,

  Roles Resources, Writing – review & editing

  Affiliation Animal Parasitic Diseases Laboratory, Agriculture Research Service, U.S. Department of Agriculture, Beltsville, Maryland, United States of America

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• Aminata Kilungo,

  Roles Writing – review & editing

  Affiliation School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America

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• Margarethe A. Cooper,

  Roles Writing – review & editing

  Affiliation School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America

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• Kerry K. Cooper,

  Roles Writing – review & editing

  Affiliations School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America, BIO5 Institute, The University of Arizona, Tucson, Arizona, United State of America

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• Gerardo U. Lopez

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: lopezg3@arizona.edu

  Affiliation School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, Arizona, United States of America

  https://orcid.org/0000-0001-7898-2129

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