Orientation of Gp96 and Calreticulin T-cell epitopes in a multiepitope HPV16 E7 vaccine construct affects predicted immunostimulatory properties: An in silico and expression validation study

• Giti Esmail Nia,

  Roles Conceptualization, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, Tehran, Iran, Department of Basic Oncology, Health Institute of Ege University, Izmir, Turkey

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• Zahra Shahosseini,

  Roles Conceptualization, Formal analysis, Methodology, Software, Writing – original draft, Writing – review & editing

  Affiliations Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, Tehran, Iran, Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran

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• Elahe Akbari,

  Roles Conceptualization, Formal analysis, Methodology, Software, Visualization

  Affiliation Department of Hepatitis, AIDS and Blood-borne Diseases, Pasteur Institute of Iran, Tehran, Iran

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• Hadi Bamehr,

  Roles Formal analysis, Methodology, Software, Validation, Visualization

  Affiliation Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

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• Fatemeh Heidarnejad,

  Roles Data curation, Software, Validation, Visualization

  Affiliation Department of Hepatitis, AIDS and Blood-borne Diseases, Pasteur Institute of Iran, Tehran, Iran

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• Monireh Mohsenzadegan,

  Roles Conceptualization, Data curation, Funding acquisition, Supervision, Validation, Writing – review & editing

  Affiliation Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

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• Azam Bolhassani ,

  Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: azam.bolhassani@yahoo.com (AB); m.farajollahi@gmail.com (MF)

  Affiliation Department of Hepatitis, AIDS and Blood-borne Diseases, Pasteur Institute of Iran, Tehran, Iran

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• Mohammadmorad Farajollahi

  Roles Data curation, Funding acquisition, Investigation, Supervision, Writing – review & editing

  * E-mail: azam.bolhassani@yahoo.com (AB); m.farajollahi@gmail.com (MF)

  Affiliation Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, Tehran, Iran

  https://orcid.org/0009-0003-1497-5140

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