Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita

Lukram Sushil Singh; Porkodi Murugesan; Gowhar Iqbal; Nagavara C. Nidarshan; Harini Gunasekaran; Ad. Viralkumar; Kiran D. Rasal; Manoj P. Brahmane; Sunil K. Nayak; Arvind A. Sonwane; Mukunda Goswami

doi:10.1371/journal.pone.0352166

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Open Access

Peer-reviewed

Research Article

Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita

Lukram Sushil Singh,

Roles Data curation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India

https://orcid.org/0009-0009-3557-848X

⨯
Porkodi Murugesan,

Roles Methodology, Software

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Gowhar Iqbal,

Roles Formal analysis, Writing – review & editing

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Nagavara C. Nidarshan,

Roles Data curation, Formal analysis

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Harini Gunasekaran,

Roles Formal analysis

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Ad. Viralkumar,

Roles Writing – review & editing

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Kiran D. Rasal,

Roles Validation, Visualization

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Manoj P. Brahmane,

Roles Validation, Visualization

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Sunil K. Nayak,

Roles Formal analysis

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Arvind A. Sonwane ,

Roles Conceptualization, Supervision, Writing – review & editing

* E-mail: arvind@cife.edu.in

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯
Mukunda Goswami

Roles Formal analysis

Affiliation Division of Fish Genetics and Biotechnology, ICAR-Central Institute of Fisheries Education, Mumbai, Maharashtra, India
⨯

Design and in silico validation of donor DNA for RNA-guided recombinase-mediated knockout of mstnb gene in Labeo rohita

Lukram Sushil Singh,
Porkodi Murugesan,
Gowhar Iqbal,
Nagavara C. Nidarshan,
Harini Gunasekaran,
Ad. Viralkumar,
Kiran D. Rasal,
Manoj P. Brahmane,
Sunil K. Nayak,
Arvind A. Sonwane

Published: July 15, 2026
https://doi.org/10.1371/journal.pone.0352166

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Protein structure prediction
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Gene targeting
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Protein domains
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Nucleotide sequencing
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DNA
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DNA repair
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