Multiplex immunofluorescence microscopy assays for pharmacodynamic assessment of MET tyrosine kinase activation in the plasma membrane and nucleus

• Tony Navas ,

  Contributed equally to this work with: Tony Navas, Apurva K. Srivastava

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  Current address: Precision Medicine (Oncology), AbbVie, Inc., North Chicago, Illinois 60064, USA

  Affiliation Clinical Pharmacodynamic Biomarkers Program, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

  https://orcid.org/0000-0002-9433-2617

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• Apurva K. Srivastava ,

  Contributed equally to this work with: Tony Navas, Apurva K. Srivastava

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: srivastavaa4@mail.nih.gov

  Affiliation Clinical Pharmacodynamic Biomarkers Program, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

  ⨯
• Scott M. Lawrence,

  Roles Investigation, Methodology, Writing – review & editing

  Current address: Cancer Genomics Research Laboratory, Leidos Biomedical Research, Inc., Frederick, Maryland 21702, USA

  Affiliation Clinical Pharmacodynamic Biomarkers Program, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

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• Melinda G. Hollingshead,

  Roles Project administration, Resources, Supervision, Writing – review & editing

  Affiliation Biological Testing Branch, Developmental Therapeutics Program, National Cancer Institute at Frederick, Frederick, Maryland, United States of America

  ⨯
• Sergio Y. Alcoser,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Biological Testing Branch, Developmental Therapeutics Program, National Cancer Institute at Frederick, Frederick, Maryland, United States of America

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• Sarah B. Miller,

  Roles Formal analysis, Writing – original draft, Writing – review & editing

  Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America

  https://orcid.org/0000-0002-0556-4003

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• Robert J. Kinders,

  Roles Conceptualization, Project administration, Supervision, Writing – review & editing

  Affiliation Clinical Pharmacodynamic Biomarkers Program, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

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• Donald P. Bottaro,

  Roles Resources, Writing – review & editing

  Affiliation Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America

  https://orcid.org/0000-0002-5057-5334

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• W. Marston Linehan,

  Roles Resources, Writing – review & editing

  Affiliation Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America

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• Shivaani Kummar,

  Roles Investigation, Resources, Supervision, Writing – review & editing

  Current address: Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA

  Affiliation Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America

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• James H. Doroshow,

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, United States of America, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America

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• Ralph E. Parchment

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  Affiliation Clinical Pharmacodynamic Biomarkers Program, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America

  https://orcid.org/0009-0002-2634-7195

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