Reference gene selection for RT-qPCR normalization in the drone testis of honey bee (Apis cerana) during meiosis stages

Lifenfang Tao; Xinran Fu; Chunxiu Pang; Yanan Zhu; Jiaxing Huang

doi:10.1371/journal.pone.0347110

Loading metrics

Open Access

Peer-reviewed

Research Article

Reference gene selection for RT-qPCR normalization in the drone testis of honey bee (Apis cerana) during meiosis stages

Lifenfang Tao,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Affiliation State Key Laboratory of Resource Insects, Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Xinran Fu,

Roles Resources

Affiliation State Key Laboratory of Resource Insects, Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China
⨯
Chunxiu Pang,

Roles Investigation

Affiliations State Key Laboratory of Resource Insects, Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China, Guangxi Academy of Agricultural Sciences, Nanning, China
⨯
Yanan Zhu ,

Roles Conceptualization, Methodology, Project administration, Software, Supervision, Validation, Writing – review & editing

* E-mail: zhuyanan@caas.cn (YZ); huangjiaxing@caas.cn (JH)

Affiliation State Key Laboratory of Resource Insects, Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China

https://orcid.org/0000-0002-2628-0624

⨯
Jiaxing Huang

Roles Conceptualization, Methodology, Project administration, Supervision, Validation, Writing – review & editing

* E-mail: zhuyanan@caas.cn (YZ); huangjiaxing@caas.cn (JH)

Affiliation State Key Laboratory of Resource Insects, Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture and Rural Affairs, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China

https://orcid.org/0000-0001-5373-6108

⨯

Reference gene selection for RT-qPCR normalization in the drone testis of honey bee (Apis cerana) during meiosis stages

Lifenfang Tao,
Xinran Fu,
Chunxiu Pang,
Yanan Zhu,
Jiaxing Huang

Published: April 9, 2026
https://doi.org/10.1371/journal.pone.0347110

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Meiosis
Is the Subject Area "Meiosis" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.
Larvae
Is the Subject Area "Larvae" applicable to this article?

Thanks for your feedback.
Honey bees
Is the Subject Area "Honey bees" applicable to this article?

Thanks for your feedback.
Tubulins
Is the Subject Area "Tubulins" applicable to this article?

Thanks for your feedback.
Pupae
Is the Subject Area "Pupae" applicable to this article?

Thanks for your feedback.
Algorithms
Is the Subject Area "Algorithms" applicable to this article?

Thanks for your feedback.
Spermatogenesis
Is the Subject Area "Spermatogenesis" applicable to this article?

Thanks for your feedback.